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Removal of Tetracycline and Tetracycline Resistance Genes from Wastewater by Constructed Wetlands

Author(s): Tadashi Toyama; Pham Thanh Hien; Kazuhiro Mori

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Abstract: We investigated the removal of a common antibiotic, tetracycline (TC), and tetracycline resistance genes (tet genes) from wastewater by fill-and-drain constructed wetlands (CWs). TC (230ug/L) containing wastewater was treated in the CWs planted or non-planted with common reed (Phragmites australis). TC was removed efficiently by the planted (95.4% removal) and non-planted (87.2% removal) CWs with 1 day. The planted CWs showed higher TC removal efficiency compared to the non-planted CWs. Adsorption of TC to soil materials had a major role in TC removal in the CWs. Biodegradation of TC by native bacteria in wastewater and CW also contributed in TC removal. Five tet genes (tet C, tet M, tet O, tet Q, and tet W) were completely removed from wastewater by the planted CWs with 1 day. In 28 days sequencing batch experiments, the planted CWs completely and repeatedly removed TC from the wastewater. The planted CWs also showed a highly effective removal of tet genes from wastewater for a long-term. These results show the potential of planted CWs for use in the removal of TC and tet genes from wastewater.

DOI:

Year: 2018

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