Author(s): Yuemei Li; Lixin He; Beibei Chai; Tianyu Zhuo; Kehong Yu; Zhixuan Zhou; Xiaohui Lei; Bin Chen

Linked Author(s): Xiaohui Lei

Keywords: Metagenomics; Hydrostatic pressure; Microbial community structure; Anaerobic hydrogen production; Sediment

Abstract: This study utilizes microcosm simulation experiments combined with metagenomic technology to investigate the impact mechanisms of four static water pressure conditions (atmospheric pressure, 0.2 MPa, 0.5 MPa, and 0.7 MPa) on hydrogen production from reservoir sediment at the microbial community structure, functional gene, and metabolic pathway levels. The results indicate that compared to atmospheric pressure, an increase in static water pressure leads to an initial increase and subsequent decrease in the total number of hydrogen-producing bacterial species in the sediment. The relative abundance of bacteria and fungi decreases by an average of 0.17% and 0.02%, respectively, while the proportion of archaea increases by an average of 0.19%. Furthermore, high pressure tightens microbial community ecological network connections, enhancing system stability. High static water pressure increases the relative abundance of functional genes related to glycolysis pathways and acetate metabolism, such as glK, ppgK, fadJ, atoA, and gene-enzyme synthesis related to butyric acid metabolism, promoting glycolysis pathways, acetate metabolism, and butyric acid metabolism pathways, thus unlocking more hydrogen release potential. These findings indicate the hydrogen production potential of reservoir sediment via anaerobic fermentation.

DOI: https://doi.org/10.3850/iahr-hic2483430201-48

Year: 2024